Elevated CDR1as may affect CACNG5 and EGFR via hsa-miR-7-5p sponge in childhood dilated cardiomyopathy patients.

Esmaiel, Nora N.; LA., Ibrahim; Fayez, Alaaeldin; Ammar, Tamer H.A.; El-Bassyouni, Hala T.

doi:10.21608/MXE.2024.377823

Elevated CDR1as may affect CACNG5 and EGFR via hsa-miR-7-5p sponge in childhood dilated cardiomyopathy patients.

Document Type : Original Article

Authors

¹ Molecular Genetics and Enzymology Department, Human Genetics and Genome Research Institute, National Research Centre, Giza, Egypt.

² Faculty of Medicine, Cairo University, Cairo, Egypt.

³ Molecular Genetics and Enzymology Department, Human Genetics and Genome Research Institute, National Research Centre, Giza, EGYPT.

⁴ Medical Molecular Genetics Department, Human Genetics and Genome Research Institute, National Research Centre, Giza, EGYPT.

⁵ Clinical Genetics Department, Human Genetics and Genome Research Institute, National Research Centre, Giza, EGYPT.

10.21608/MXE.2024.377823

Abstract

Background: In the present study, we mainly aimed to evaluate CDR1as and hsa-circRNA_105039 biomarkers expression in childhood dilated cardiomyopathy (DCM) and ventricular septal defects (VSD) patients. Circular RNAs (circRNAs) are non-coding RNAs that result from the back splicing of pre-mRNA. Circular RNA's exhibit enhanced stability with numerous biological functions. Thus, this circRNAs are promising targets for developing diagnostic tools and therapies for the human diseases. Methods: Fold change of CDR1as and hsa-circRNA_105039 was detected by qRT-PCR in 101 participants. The diagnostic accuracy of CDR1as was determined using receiver operating characteristic curve analysis. To predict CDR1as/miRNAs and CDR1as/proteins interaction networks related to DCM and VSD pathogenesis, gene ontology (GO) and KEGG pathway analyses were performed. Results: CDR1as showed significant higher fold change (FC= 2.9) in DCM group than both control and VSD groups. Experimental evidence-based GO and KEGG pathways analyses showed that CDR1as has 73 miRNAs binding sites on hsa-miR-7-5p which targets 3'UTR of mRNAs involved in MAPK signaling pathway. Conclusion: The potential molecular mechanistic effect of the elevated CDR1as could be concluded by sponging of hsa-miR-7-5p in childhood DCM patients. Consequently, further decreasing of hsa-miR-7-5p may lead to increased dosage of CACNG5 and EGFR which involved in the MAPK signaling pathway.

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